Специфичность
ab54223 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. ab54223 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6C hi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C + exudate macrophages, whereas more mature Ly-6C -/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state. ab54223 was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2