Название
Anti-p53 antibody [E47]
Applications
ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP
Обьем
20 µL; 100 µL; 1 mL
Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Концентрация
0.122 mg/mL Batch dependent concentration
Подтвержденная реактивность
Human
Специфичность
This antibody clone recognises both wild-type and mutant forms of p53 in human samples. It is not designed to recognise any specific p53 mutation. We have confirmed this experimentally and have been able to detect p53 in different cell lines using various applications and treatments. Important note: p53 expression levels vary greatly between cell lines. It has been reported that p53 mutations render the protein more stable, hence mutated cell lines often express higher levels of the p53 protein compared to wild-type cell lines. For low expressing wild type cell lines, p53 expression can be increased with cell treatments such as camptothecin or irinotecan.